Supplementary Materials

Supplementary Materials for:

MAFB enhances oncogenic Notch signaling in T cell acute lymphoblastic leukemia

Kostandin V. Pajcini,* Lanwei Xu, Lijian Shao, Jelena Petrovic, Karol Palasiewicz, Yumi Ohtani, Will Bailis, Curtis Lee, Gerald B. Wertheim, Rajeswaran Mani, Natarajan Musuthamy, Yunlei Li, Jules P. P. Meijerink, Stephen C. Blacklow, Robert B. Faryabi, Sara Cherry, Warren S. Pear*

*Corresponding author. Email: kvp{at}uic.edu (K.V.P.); wpear{at}mail.med.upenn.edu (W.S.P.)

This PDF file includes:

  • Fig. S1. Validation of Notch1 gain-of-function screen and MAFB expression in murine and human T-ALL.
  • Fig. S2. Analysis of spleen and peripheral blood after BMT.
  • Fig. S3. Validation of MafB siRNA and shRNA reagents.
  • Fig. S4. Ectopic MAFB expression enhances T6E cell growth.
  • Fig. S5. MAFB interacts with DNA through ETS factors, and the loss of ETS1 delays onset of T-ALL.
  • Fig. S6. Zmiz1 expression in T6E cells is not affected by the loss of MafB.
  • Legends for tables S1 and S2
  • Table S3. List of oligo sequences and primer sets for qPCR and OIP.

[Download PDF]

Technical Details

Format: Adobe Acrobat PDF

Size: 1.88 MB

Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). Notch signaling enhancement of Notch mutant LPΔP by candidate genes from the cDNA library.
  • Table S2 (Microsoft Excel format). MafB suppression affects a subset of Notch signaling gene targets in T6E cells.

[Download Tables S1 and S2]


Citation: K. V. Pajcini, L. Xu, L. Shao, J. Petrovic, K. Palasiewicz, Y. Ohtani, W. Bailis, C. Lee, G. B. Wertheim, R. Mani, N. Musuthamy, Y. Li, J. P. P. Meijerink, S. C. Blacklow, R. B. Faryabi, S. Cherry, W. S. Pear, MAFB enhances oncogenic Notch signaling in T cell acute lymphoblastic leukemia. Sci. Signal. 10, eaam6846 (2017).

© 2017 American Association for the Advancement of Science