Supplementary Materials

Supplementary Materials for:

ATP promotes the fast migration of dendritic cells through the activity of pannexin 1 channels and P2X7 receptors

Pablo J. Sáez,* Pablo Vargas, Kenji F. Shoji, Paloma A. Harcha, Ana-María Lennon-Duménil,* Juan C. Sáez*

*Corresponding author. Email: pablo.saez{at}curie.fr (P.J.S.); ana-maria.lennon{at}curie.fr (A.-M.L.-D.); jsaez{at}bio.puc.cl (J.C.S.)

This PDF file includes:

  • Fig. S1. Extracellular ATP increases the permeability of the plasma membrane in DCs to DAPI.
  • Fig. S2. The abundances of Panx2, Panx3, Cx43, and Cx45 are similar between WT and Panx1−/− DCs.
  • Fig. S3. ATP-induced dye uptake by DCs is selective.
  • Fig. S4. Extracellular ATP contributes to migration of DCs.
  • Fig. S5. WT and Panx1−/− DCs have functional P2Y receptors that do not contribute to ATP-induced migration or membrane permeabilization.
  • Fig. S6. ATP stimulates the migration, but not the death, of DCs.
  • Fig. S7. Role of extracellular Ca2+ and Cx hemichannels in DC migration.
  • Fig. S8. ATP-induced reorganization of the actin cytoskeleton.
  • Fig. S9. The ATP-induced cell surface expression of CD40 and CD86 is Panx-independent.
  • Legend for movies S1 to S5

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.avi format). WT and Panx1−/− DCs in a representative DAPI uptake experiment.
  • Movie S2 (.avi format). WT and Panx1−/− DCs migrating inside microchannels under resting conditions or after exposure to ATP.
  • Movie S3 (.avi format). WT LifeAct-GFP DCs migrating inside microchannels.
  • Movie S4 (.avi format). ATP-pulsed WT LifeAct-GFP DCs migrating inside microchannels.
  • Movie S5 (.mp4 format). ATP-pulsed WT and Panx1−/− DCs migrating randomly in collagen gels.

Citation: P. J. Sáez, P. Vargas, K. F. Shoji, P. A. Harcha, A.-M. Lennon-Duménil, J. C. Sáez, ATP promotes the fast migration of dendritic cells through the activity of pannexin 1 channels and P2X7 receptors. Sci. Signal. 10, eaah7107 (2017).

© 2017 American Association for the Advancement of Science