Supplementary Materials for:

Microtubules tune mechanotransduction through NOX2 and TRPV4 to decrease sclerostin abundance in osteocytes

James S. Lyons, Humberto C. Joca, Robert A. Law, Katrina M. Williams, Jaclyn P. Kerr, Guoli Shi, Ramzi J. Khairallah, Stuart S. Martin, Konstantinos Konstantopoulos, Christopher W. Ward,* Joseph P. Stains*

*Corresponding author. Email: jstains{at}som.umaryland.edu (J.P.S.); ward{at}som.umaryland.edu (C.W.W.)

This PDF file includes:

• Fig. S1. Control and PTL/Taxol-treated Ocy454 cells show indistinguishable FSS-induced Ca²⁺ responses at 16 dynes/cm².
• Fig. S2. Statistical significance of treatment groups in Fig. 6.
• Fig. S3. The Ca²⁺ channel TRPV4 is abundant at the mRNA level in Ocy454 cells.
• Fig. S4. Increased FSS does not rescue FSS-induced Ca²⁺ influx in Ocy454 cells treated with α-NAC or GP91ds-TAT, and TRPV4 activation does not affect ROS production.

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Citation: J. S. Lyons, H. C. Joca, R. A. Law, K. M. Williams, J. P. Kerr, G. Shi, R. J. Khairallah, S. S. Martin, K. Konstantopoulos, C. W. Ward, J. P. Stains, Microtubules tune mechanotransduction through NOX2 and TRPV4 to decrease sclerostin abundance in osteocytes. Sci. Signal. 10, eaan5748 (2017).

© 2017 American Association for the Advancement of Science