Supplementary Materials for:

Nuclear hyaluronidase 2 drives alternative splicing of CD44 pre-mRNA to determine profibrotic or antifibrotic cell phenotype

Adam C. Midgley, Sebastian Oltean, Vincent Hascall, Emma L. Woods, Robert Steadman, Aled O. Phillips, Soma Meran*

*Corresponding author. Email: merans{at}cf.ac.uk

This PDF file includes:

• Fig. S1. Schematic and confirmation of function for pRG6-CD44v bichromatic minigene reporters.
• Fig. S2. In silico analysis of intronic sequence surrounding exons 11 and 12 of CD44v7 and CD44v8.
• Fig. S3. Confirmation of SRSF2 and SRSF5 knockdown by Western blot.
• Fig. S4. SRSF2 RIP.
• Table S1. Threshold scores for SR proteins (CD44 introns 10, 11, and 12) derived from in silico analysis.
• Table S2. Threshold scores for SR proteins (CD44 exons 11 and 12) derived from in silico analysis.
• Table S3. Individual protein IDs from nuclear HYAL2 coimmunoprecipitation.
• Table S4. In silico analysis of HYAL2 splice factor binding site thresholds.
• Table S5. Primer sequences used for qRT-PCR and RIP.
• Table S6. qRT-PCR cycle threshold values for CD44s and CD44v7/8.

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Citation: A. C. Midgley, S. Oltean, V. Hascall, E. L. Woods, R. Steadman, A. O. Phillips, S. Meran, Nuclear hyaluronidase 2 drives alternative splicing of CD44 pre-mRNA to determine profibrotic or antifibrotic cell phenotype. Sci. Signal. 10, eaao1822 (2017).

© 2017 American Association for the Advancement of Science