Supplementary Materials

Supplementary Materials for:

Networks of enzymatically oxidized membrane lipids support calcium-dependent coagulation factor binding to maintain hemostasis

Sarah N. Lauder, Keith Allen-Redpath, David A. Slatter, Maceler Aldrovandi, Anne O'Connor, Daniel Farewell, Charles L. Percy, Jessica E. Molhoek, Sirpa Rannikko, Victoria J. Tyrrell, Salvatore Ferla, Ginger L. Milne, Alastair W. Poole, Christopher P. Thomas, Samya Obaji, Philip R. Taylor, Simon A. Jones, Phillip G. de Groot, Rolf T. Urbanus, Sohvi Hörkkö, Stefan Uderhardt, Jochen Ackermann, P. Vince Jenkins, Andrea Brancale, Gerhard Krönke, Peter W. Collins,* Valerie B. O'Donnell*

*Corresponding author. Email: o-donnellvb{at}cardiff.ac.uk (V.B.O.); collinspw{at}cardiff.ac.uk (P.W.C.)

This PDF file includes:

  • Fig. S1. Primary data for the generation of eoxPLs by washed wild-type and ALOX12−/− platelets.
  • Fig. S2. Analysis of HC and APS demographics for effects on eoxPL generation shows no effect of age, gender, or arterial thrombosis on platelet 12-HETE-PEs.
  • Fig. S3. Primary data for the generation of eoxPLs by washed platelets from HC and APS patients.
  • Fig. S4. Correlation plots for lipids from all groups show that lipids group according to enzymatic origin.
  • Fig. S5. Correlation plots for lipids from either HC or APS patients show that lipids group according to enzymatic origin.
  • Table S1. Patient demographics and relevant medical details.
  • Table S2. Age and gender demographics of study participants.
  • Legend for movie S1

[Download PDF]

Technical Details

Format: Adobe Acrobat PDF

Size: 2.49 MB

Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.avi format). Simulation of the movement of HETE-PC in a lipid biomembrane.

Citation: S. N. Lauder, K. Allen-Redpath, D. A. Slatter, M. Aldrovandi, A. O'Connor, D. Farewell, C. L. Percy, J. E. Molhoek, S. Rannikko, V. J. Tyrrell, S. Ferla, G. L. Milne, A. W. Poole, C. P. Thomas, S. Obaji, P. R. Taylor, S. A. Jones, P. G. de Groot, R. T. Urbanus, S. Hörkkö, S. Uderhardt, J. Ackermann, P. Vince Jenkins, A. Brancale, G. Krönke, P. W. Collins, V. B. O'Donnell, Networks of enzymatically oxidized membrane lipids support calcium-dependent coagulation factor binding to maintain hemostasis. Sci. Signal. 10, eaan2787 (2017).

© 2017 American Association for the Advancement of Science