Supplementary Materials

Supplementary Materials for:

Transmembrane helix connectivity in Orai1 controls two gates for calcium-dependent transcription

Irene Frischauf, Monika Litviňuková, Romana Schober, Vasilina Zayats, Barbora Svobodová, Daniel Bonhenry, Victoria Lunz, Sabrina Cappello, Laura Tociu, David Reha, Amrutha Stallinger, Anna Hochreiter, Teresa Pammer, Carmen Butorac, Martin Muik, Klaus Groschner, Ivan Bogeski, Rüdiger H. Ettrich, Christoph Romanin, Rainer Schindl*

*Corresponding author. Email: rainer.schindl{at}medunigraz.at

This PDF file includes:

  • Fig. S1. Constitutive activity and cellular localization of cancer database and myopathy Orai1 mutants.
  • Fig. S2. Interaction between TM2 and pore helix residues.
  • Fig. S3. STIM1 and Orai1-His134 mutants mediate Ca2+-selective currents.
  • Fig. S4. Starvation induces nuclear translocation of the transcription factors MITF and TFEB.
  • Fig. S5. Pharmacological regulation and fast inactivation of the Orai1-H134A mutant.
  • Fig. S6. Pore profile of human and Drosophila Orai.
  • Fig. S7. Regulation of the Arg91 gate in closed and constitutively open Orai1.
  • Fig. S8. Pulled Ca2+ ion through the Orai1 pore extends the Arg91 gate.
  • Fig. S9. The hydrophobic gate forms a barrier for water molecules in Orai1 channels.
  • Fig. S10. Cysteine cross-linking of constitutively active Orai1 mutants.
  • Fig. S11. Dynamics of water molecules in closed and constitutively active Orai1 channels.
  • Table S1. Selectivity of Orai1 mutants.
  • Legend for movie S1

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.avi format). Ca2+ permeation through the Orai1 channel.

Citation: I. Frischauf, M. Litviňuková, R. Schober, V. Zayats, B. Svobodová, D. Bonhenry, V. Lunz, S. Cappello, L. Tociu, D. Reha, A. Stallinger, A. Hochreiter, T. Pammer, C. Butorac, M. Muik, K. Groschner, I. Bogeski, R. H. Ettrich, C. Romanin, R. Schindl, Transmembrane helix connectivity in Orai1 controls two gates for calcium-dependent transcription. Sci. Signal. 10, eaao0358 (2017).

© 2017 American Association for the Advancement of Science