Supplementary Materials

Supplementary Materials for:

Subtle modifications to oxytocin produce ligands that retain potency and improved selectivity across species

Markus Muttenthaler,* Åsa Andersson, Irina Vetter, Rohit Menon, Marta Busnelli, Lotten Ragnarsson, Christian Bergmayr, Sarah Arrowsmith, Jennifer R. Deuis, Han Sheng Chiu, Nathan J. Palpant, Margaret O'Brien, Terry J. Smith, Susan Wray, Inga D. Neumann, Christian W. Gruber, Richard J. Lewis, Paul F. Alewood*

*Corresponding author. Email: markus.muttenthaler{at}univie.ac.at, markus.muttenthaler{at}univie.ac.at (M.M.);
p.alewood{at}uq.edu.au (P.F.A.)

This PDF file includes:

  • Materials and Methods
  • Fig. S1. Representative raw FLIPR data.
  • Fig. S2. Schild plot analysis.
  • Fig. S3. Functional study of [Se-Se]-OT-OH and d[Se-Se]-OT-OH at the mOTR, mV1aR, mV1bR, and mV2R.
  • Fig. S4. Binding study of [Se-Se]-OT-OH and d[Se-Se]-OT-OH at the mOTR, mV1aR, mV1bR, and mV2R.
  • Fig. S5. Human myometrial cell contractility assay.
  • Fig. S6. Human myometrial strip contractility assay.
  • Table S1. Overview of the synthesized peptides including details on their modifications.
  • Table S2. Functional potencies for oxytocin and vasopressin analogs at the hOTR, hV1aR, hV1bR, and hV2R.
  • Table S3. Functional potencies for oxytocin and vasopressin analogs at the human and murine OTR, V1aR, V1bR, and V2R.
  • Table S4. Comparison of functional data from the FLIPR and HTRF-IP1 assays.
  • Table S5. Radioligand displacement data for oxytocin and vasopressin analogs at the human and murine OTR, V1aR, V1bR, and V2R.
  • Table S6. Overview of the number of electric foot shocks per mouse.
  • Reference (110)

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Citation: M. Muttenthaler, Å. Andersson, I. Vetter, R. Menon, M. Busnelli, L. Ragnarsson, C. Bergmayr, S. Arrowsmith, J. R. Deuis, H. S. Chiu, N. J. Palpant, M. O’Brien, T. J. Smith, S. Wray, I. D. Neumann, C. W. Gruber, R. J. Lewis, P. F. Alewood, Subtle modifications to oxytocin produce ligands that retain potency and improved selectivity across species. Sci. Signal. 10, eaan3398 (2017).

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