Supplementary Materials

Supplementary Materials for:

The receptor tyrosine kinase EphA2 promotes glutamine metabolism in tumors by activating the transcriptional coactivators YAP and TAZ

Deanna N. Edwards, Verra M. Ngwa, Shan Wang, Eileen Shiuan, Dana M. Brantley-Sieders, Laura C. Kim, Albert B. Reynolds, Jin Chen*

*Corresponding author. Email: jin.chen{at}vanderbilt.edu

This PDF file includes:

  • Fig. S1. EphA2 activates YAP/TAZ in MCF10A-HER2 cells.
  • Fig. S2. EphA2–ephrin-A1 interactions do not significantly contribute to YAP/TAZ activation.
  • Fig. S3. Rho and ROCK inhibition did not enhance LATS1 phosphorylation in MMTV-Neu cells.
  • Fig. S4. EphA2-mediated YAP/TAZ activation requires Rho catalytic activity.
  • Fig. S5. YAP and TAZ promote glutamine metabolism in MCF10A-HER2-EphA2 cells.
  • Fig. S6. YAP and TEAD4 are associated with GLS and SLC1A5 promoters.
  • Fig. S7. YAP/TAZ and EphA2 expression strongly correlates with decreased patient survival in HER2-positive breast cancer.
  • Table S1. ChIP primers.
  • Legends for movies S1 and S2

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mov format). EphA2 overexpression leads to intranuclear accumulation of YAP.
  • Movie S2 (.mov format). Nuclear exclusion of YAP upon Rho inhibition.

Citation: D. N. Edwards, V. M. Ngwa, S. Wang, E. Shiuan, D. M. Brantley-Sieders, L. C. Kim, A. B. Reynolds, J. Chen, The receptor tyrosine kinase EphA2 promotes glutamine metabolism in tumors by activating the transcriptional coactivators YAP and TAZ. Sci. Signal. 10, eaan4667 (2017).

© 2017 American Association for the Advancement of Science