Supplementary Materials

Supplementary Materials for:

RhoB controls the Rab11-mediated recycling and surface reappearance of LFA-1 in migrating T lymphocytes

Malin Samuelsson, Katarzyna Potrzebowska, Janne Lehtonen, Jason P. Beech, Ekatarina Skorova, Heli Uronen-Hansson, Lena Svensson*

*Corresponding author. Email: lena_m.svensson{at}med.lu.se

This PDF file includes:

  • Fig. S1. RhoB interacts with LFA-1, and a reduction in RhoB abundance impairs LFA-1–dependent migration in T lymphocytes.
  • Fig. S2. LFA-1 is present in cytosolic clusters similar to those containing RhoB and tubulin in migrating T lymphocytes.
  • Fig. S3. Both LFA-1 and RhoB interact with Rab11 in T lymphocytes.
  • Fig. S4. Active RhoB directs LFA-1 between cytoplasmic and membrane compartments in HSB-2 cells.
  • Fig. S5. Decreased abundance of functional RhoB impairs LFA-1 internalization and recycling.
  • Fig. S6. RhoB activity regulates Rab11 localization, and both proteins control LFA-1 recycling in HSB-2 cells.

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Citation: M. Samuelsson, K. Potrzebowska, J. Lehtonen, J. P. Beech, E. Skorova, H. Uronen-Hansson, L. Svensson, RhoB controls the Rab11-mediated recycling and surface reappearance of LFA-1 in migrating T lymphocytes. Sci. Signal. 10, eaai8629 (2017).

© 2017 American Association for the Advancement of Science