Supplementary Materials

Supplementary Materials for:

Aging impairs both primary and secondary RIG-I signaling for interferon induction in human monocytes

Ryan D. Molony, Jenny T. Nguyen, Yong Kong, Ruth R. Montgomery, Albert C. Shaw, Akiko Iwasaki*

*Corresponding author. Email: akiko.iwasaki{at}yale.edu

This PDF file includes:

  • Fig. S1. Monocyte purity and plasmacytoid DC numbers are comparable between age groups.
  • Fig. S2. IFN Induction in response to dsRNA transfection depends on a 5′-ppp motif in human monocytes.
  • Fig. S3. Basal IFNB expression is comparable in human monocytes from older and younger donors.
  • Fig. S4. IFN-β pretreatment is insufficient to ablate age-related differences in IFN-β secretion upon RIG-I stimulation.
  • Fig. S5. Basal levels of RIG-I, IRF3, and IRF7 protein are comparable in older and younger monocytes.
  • Fig. S6. IRF3 phosphorylation is impaired in monocytes from older donors.
  • Fig. S7. Basal TRAF3 protein levels are positively correlated with IFN-β secretion in response to RIG-I stimulation.
  • Fig. S8. Bortezomib improves antiviral responses in monocytes from older donors.
  • Fig. S9. Lack of age-related differences in IFN-β secretion in response to the cGAMP stimulation of human monocytes.
  • Fig. S10. RNA-seq highlights the differential regulation of RIG-I, IRF, and IAVrelated signaling pathways in older RIG-I–stimulated monocytes.
  • Fig. S11. Amplification-stage increase in IRF7 protein is defective in older RIGI–stimulated monocytes.
  • Fig. S12. Basal IRF8 protein and RNA levels are comparable in old and young monocytes.
  • Fig. S13. Inducible IRF8 expression is age-dependent.
  • Fig. S14. Inducible IRF8 levels are positively correlated with RIG-I–inducible IFN-β secretion.
  • Fig. S15. Bortezomib treatment of older monocytes enhances IRF8 induction upon RIG-I stimulation.
  • Fig. S16. IFN-β–stimulated gene induction and inducible protection from influenza is preserved with age in human monocytes.
  • Fig. S17. IRF8 is an IFN-inducible gene, and its induction is impaired in older monocytes.
  • Fig. S18. Lack of age-related difference in CpG DNA methylation of the IRF8 gene in human monocytes.
  • Table S1. Human donor characteristics.
  • Table S2. Differences in IFN expression in the RNA-seq data from RIG-I–stimulated older and younger monocytes.
  • Table S3. Differences in IRF expression in the RNA-seq data from RIG-I–stimulated older and younger monocytes.

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Citation: R. D. Molony, J. T. Nguyen, Y. Kong, R. R. Montgomery, A. C. Shaw, A. Iwasaki, Aging impairs both primary and secondary RIG-I signaling for interferon induction in human monocytes. Sci. Signal. 10, eaan2392 (2017).

© 2017 American Association for the Advancement of Science