Supplementary Materials

Supplementary Materials for:

Oncogenic PI3K promotes methionine dependency in breast cancer cells through the cystine-glutamate antiporter xCT

Evan C. Lien, Laura Ghisolfi, Renee C. Geck, John M. Asara, Alex Toker*

*Corresponding author. Email: atoker{at}bidmc.harvard.edu

This PDF file includes:

  • Fig. S1. Characterization of MCF10A cells stably expressing PIK3CA variants.
  • Fig. S2. SLC7A11 and MAT1A expression correlate with methionine dependency in breast cancer cell lines.
  • Fig. S3. Endogenous xCT phosphorylation is inhibited by PI3K and AKT inhibitors.
  • Fig. S4. xCT functionally contributes to the methionine dependency phenotype.
  • Fig. S5. Inhibition of AKT signaling by GDC-0941 during metabolic labeling with [U-13C5]-methionine.
  • Legend for Data File S1

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Other Supplementary Material for this manuscript includes the following:

  • Data File S1 (Microsoft Excel format). Spearman rank correlation between methionine dependency and expression of methionine and cysteine metabolism genes.

Citation: E. C. Lien, L. Ghisolfi, R. C. Geck, J. M. Asara, A. Toker, Oncogenic PI3K promotes methionine dependency in breast cancer cells through the cystine-glutamate antiporter xCT. Sci. Signal. 10, eaao6604 (2017).

© 2017 American Association for the Advancement of Science