Supplementary Materials

Supplementary Materials for:

The depalmitoylase APT1 directs the asymmetric partitioning of Notch and Wnt signaling during cell division

Ewa Stypulkowski, Irfan A. Asangani, Eric S. Witze*

*Corresponding author. Email: ewitze{at}exchange.upenn.edu

This PDF file includes:

  • Fig. S1. Scoring method for determining asymmetric divisions.
  • Fig. S2. DHHC20 and APT1 localization in MDA-MB-231 cells.
  • Fig. S3. Effect of CDC42 and PARD3 knockdown on asymmetric Numb and β-catenin localization.
  • Fig. S4. CDC42 activity and lipidation promote asymmetric APT1, Numb, and β-catenin localization.
  • Fig. S5. Validation of RNA-seq and additional GSEA analyses relating to Fig. 6.
  • Fig. S6. Staining of asymmetric APT1 in mouse embryonic stem cell.
  • Fig. S7. Colony counts, growth curves, and reporter expression relating to Fig. 7.
  • Fig. S8. Gating scheme for ALDH+ cells on dissociated colonies or adherent cells.
  • Legend for table S1
  • Legends for movies S1 to S10

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Other Supplementary Material for this manuscript includes the following:

  • Table S1. (Microsoft Excel format)Excel spreadsheet of RNA-seq annotated genes.
  • Movie S1 (.avi format). Single channel of a U2 OS cell ectopically expressing APT1WT-CFP (blue).
  • Movie S2 (.avi format). Single channel of a U2 OS cell from movie S1 ectopically expressing NumbWT-YFP (yellow).
  • Movie S3 (.avi format). Merge of movie S1 of a U2 OS cell ectopically expressing APT1WT-CFP (blue), NumbWT-YFP (yellow), and mCherry–Histone H2B (red).
  • Movie S4 (.avi format). Single channel of a U2 OS cell ectopically expressing APT1WT-CFP (blue).
  • Movie S5 (.avi format). Merge of movie S4 of a U2 OS cell ectopically expressing APT1WT-CFP (blue) and mCherry–Histone H2B (red).
  • Movie S6 (.avi format). Single channel of a U2 OS cell ectopically expressing APT1S119A-CFP (blue).
  • Movie S7 (.avi format). Merge of movie S6 of a U2 OS cell ectopically expressing APT1S119A-CFP (blue) and mCherry–Histone H2B (red).
  • Movie S8 (.avi format). Single channel of a U2 OS cell ectopically expressing APT1WT-CFP (blue).
  • Movie S9 (.avi format). Single channel of a U2 OS cell ectopically expressing YFP-CDC42Palm (yellow).
  • Movie S10 (.avi format). Merge of movie S1 of a U2 OS cell ectopically expressing APT1WT-CFP (blue), YFP-CDC42Palm (yellow), and mCherry–Histone H2B (red).

Citation: E. Stypulkowski, I. A. Asangani, E. S. Witze, The depalmitoylase APT1 directs the asymmetric partitioning of Notch and Wnt signaling during cell division. Sci. Signal. 11, eaam8705 (2018).

© 2018 American Association for the Advancement of Science