Supplementary Materials

Supplementary Materials for:

Ca2+-dependent demethylation of phosphatase PP2Ac promotes glucose deprivation–induced cell death independently of inhibiting glycolysis

Ha Yin Lee, Yoko Itahana, Stefan Schuechner, Masahiro Fukuda, H. Shawn Je, Egon Ogris, David M. Virshup, Koji Itahana*

*Corresponding author. Email: koji.itahana{at}duke-nus.edu.sg

This PDF file includes:

  • Fig. S1. Glucose deprivation induces cell death in a subset of cancer cells.
  • Fig. S2. 2-DG rescues glucose deprivation–induced cell death that is independent from ROS induction.
  • Fig. S3. 2-DG rescues glucose deprivation–induced cell death independently from O-glycosylation.
  • Fig. S4. Glucose deprivation–induced cell death is not mediated by apoptosis.
  • Fig. S5. Glucose deprivation–induced cell death is independent from autophagy.
  • Fig. S6. AMPK is not involved in glucose deprivation–induced cell death.
  • Fig. S7. Glucose deprivation induces RIPK1 phosphorylation.
  • Fig. S8. Glucose deprivation induces RIPK1-dependent cell death.
  • Fig. S9. Glucose deprivation–induced cell death is not mediated by necroptosis.
  • Fig. S10. Glucose deprivation induces PP2Ac demethylation.
  • Fig. S11. Glucose deprivation induces PP2Ac demethylation and RIPK1 phosphorylation independently from the mTOR signaling pathway.
  • Fig. S12. PP2Ac demethylation is required for glucose deprivation–induced cell death.
  • Fig. S13. Glucose deprivation induces calcium influx into the cytoplasm.
  • Fig. S14. Knockdown efficiency of CACNA1C and CACNA1D.
  • Fig. S15. GLUT1 inhibitor increases cytoplasmic calcium concentration in U2OS cells.
  • Legends for movies S1 to S8

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mp4 format). PI staining in the presence of glucose.
  • Movie S2 (.mp4 format). PI staining in the absence of glucose.
  • Movie S3 (.mp4 format). Cell death in the presence of control siRNA and glucose.
  • Movie S4 (.mp4 format). Cell death in the presence of PPME1 siRNA and glucose.
  • Movie S5 (.mp4 format). Cell death in the presence of control siRNA but in the absence of glucose.
  • Movie S6 (.mp4 format). Cell death in the presence of PPME1 siRNA but in the absence of glucose.
  • Movie S7 (.mp4 format). Calcium staining in the presence of glucose.
  • Movie S8 (.mp4 format). Calcium staining in the absence of glucose.

Citation: H. Y. Lee, Y. Itahana, S. Schuechner, M. Fukuda, H. S. Je, E. Ogris, D. M. Virshup, K. Itahana, Ca2+-dependent demethylation of phosphatase PP2Ac promotes glucose deprivation– induced cell death independently of inhibiting glycolysis. Sci. Signal. 11, eaam7893 (2018).

© 2018 American Association for the Advancement of Science