Supplementary Materials

Supplementary Materials for:

Biased signaling by thyroid-stimulating hormone receptor–specific antibodies determines thyrocyte survival in autoimmunity

Syed A. Morshed,* Risheng Ma, Rauf Latif, Terry F. Davies

*Corresponding author. Email: syed.morshed{at}mssm.edu

This PDF file includes:

  • Fig. S1. The C-TSHR-mAb disrupts vesicular trafficking.
  • Fig. S2. The C-TSHR-mAb does not stimulate signaling in CHO-TSHR cells.
  • Fig. S3. Effects of G protein perturbations on Gαs, Gαq, Gβγ, and Gα12 activities on CHO-TSHR cells.
  • Fig. S4. The Ras-PI3K-Rac pathway downstream of G proteins is a key signaling pathway for vesicle formation.
  • Fig. S5. Perturbation of major G proteins by different pharmacological inhibitors in CHO-TSHR cells.
  • Fig. S6. Dose-dependent suppression of the TSH-induced activities of Gαs, Gαq, Gβγ, and Gα12 by pharmacological inhibitors in CHO-TSHR cells.
  • Fig. S7. Model depicting the mechanism of action of C-TSHR-mAb in FRTL-5 thyrocytes.
  • Fig. S8. The C-TSHR-mAb induces apoptosis in thyrocytes and fibroblasts.

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Citation: S. A. Morshed, R. Ma, R. Latif, T. F. Davies, Biased signaling by thyroid-stimulating hormone receptor–specific antibodies determines thyrocyte survival in autoimmunity. Sci. Signal. 11, eaah4120 (2018).

© 2018 American Association for the Advancement of Science