Supplementary Materials

Supplementary Materials for:

Guanabenz inhibits TLR9 signaling through a pathway that is independent of eIF2α dephosphorylation by the GADD34/PP1c complex

Jessica Perego, Andreia Mendes, Clarisse Bourbon, Voahirana Camosseto, Alexis Combes, Hong Liu, Thien-Phong Vu Manh, Alexandre Dalet, Lionel Chasson, Lionel Spinelli, Nathalie Bardin, Laurent Chiche, Manuel A. S. Santos, Evelina Gatti,* Philippe Pierre*

*Corresponding author. Email: gatti{at}ciml.univ-mrs.fr (E.G.); pierre{at}ciml.univ-mrs.fr (P.P.)

This PDF file includes:

  • Fig. S1. GBZ inhibits TLR9 signaling in B cells.
  • Fig. S2. TMPD and CpG synergize to promote inflammation.
  • Fig. S3. GBZ does not inhibit endosomal acidification or protein synthesis.
  • Fig. S4. GBZ and 25-HC do not reduce TLR9 abundance in pDCs.
  • Table S1. List of genes in DCs with increased expression in response to poly(I:C) or P. mirabilis that is inhibited by GBZ.
  • Table S2. List of the primers used for gene expression analysis by qPCR.
  • Table S3. List of the antibodies used for sorting DC1, DC2, and pDCs from Flt3L-DC culture.
  • Table S4. List of the antibodies used for flow cytometry.

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Citation: J. Perego, A. Mendes, C. Bourbon, V. Camosseto, A. Combes, H. Liu, T.-P. V. Manh, A. Dalet, L. Chasson, L. Spinelli, N. Bardin, L. Chiche,M. A. S. Santos, E. Gatti, P. Pierre, Guanabenz inhibits TLR9 signaling through a pathway that is independent of eIF2α dephosphorylation by the GADD34/PP1c complex. Sci. Signal. 11, eaam8104 (2018).

© 2018 American Association for the Advancement of Science