Supplementary Materials

Supplementary Materials for:

Fluorescent Ca2+ indicators directly inhibit the Na,K-ATPase and disrupt cellular functions

Nathan A. Smith, Benjamin T. Kress, Yuan Lu, Devin Chandler-Militello, Abdellatif Benraiss, Maiken Nedergaard*

*Corresponding author. Email: nedergaard{at}urmc.rochester.edu

This PDF file includes:

  • Fig. S1. Structures and characters of Ca2+ indicator derivatives used in publications listed in PubMed.
  • Fig. S2. DMSO does not affect 86Rb+ uptake by astrocytes.
  • Fig. S3. Ca2+ indicators do not affect ouabain-insensitive 86Rb+ uptake.
  • Fig. S4. Ca2+ indicators inhibit Na,K-ATPase–mediated ATP hydrolysis and 86Rb+ uptake in rat astrocyte cultures.
  • Fig. S5. Ca2+ indicators induce lactate release in neurons and increase cell volume in neurons and astrocytes.
  • Fig. S6. Quantification of spontaneous and pharmacologically evoked Ca2+ signals and 86Rb+ uptake in GCaMP3-expressing astrocytes.
  • Legends for movies S1 to S5

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.avi format). Beating cardiomyocytes in culture prepared from mouse (2× frame rate).
  • Movie S2 (.avi format). ATP-induced Ca2+ response in astrocytes expressing GFAP-GCaMP3.
  • Movie S3 (.avi format). ATP-induced Ca2+ response in astrocytes expressing AdV-GCaMP3.
  • Movie S4 (.avi format). ATP-induced Ca2+ response in astrocytes loaded with Rhod-2 AM.
  • Movie S5 (.avi format). Spontaneous Ca2+ response in astrocytes expressing GFAP-GCaMP3.

Citation: N. A. Smith, B. T. Kress, Y. Lu, D. Chandler-Militello, A. Benraiss, M. Nedergaard, Fluorescent Ca2+ indicators directly inhibit the Na,K-ATPase and disrupt cellular functions. Sci. Signal. 11, eaal2039 (2018).

© 2018 American Association for the Advancement of Science