Supplementary Materials

Supplementary Materials for:

SILAC identifies LAD1 as a filamin-binding regulator of actin dynamics in response to EGF and a marker of aggressive breast tumors

Lee Roth, Swati Srivastava, Moshit Lindzen, Aldema Sas-Chen, Michal Sheffer, Mattia Lauriola, Yehoshua Enuka, Ashish Noronha, Maicol Mancini, Sara Lavi, Gabi Tarcic, Gur Pines, Nava Nevo, Ori Heyman, Tamar Ziv, Oscar M. Rueda, Davide Gnocchi, Eli Pikarsky, Arie Admon, Carlos Caldas, Yosef Yarden*

*Corresponding author. Email: yosef.yarden{at}weizmann.ac.il

This PDF file includes:

  • Fig. S1. LAD1 is an intracellular protein that undergoes MEK-dependent phosphorylation in response to EGF.
  • Fig. S2. Genomic, proteomic, and transcriptional characteristics of LAD1.
  • Fig. S3. Knockdown of LAD1 impairs invadopodia formation by mammary cells.
  • Fig. S4. Phosphorylation of LAD1 on specific serine sites moderately regulates cell migration and viability, without markedly affecting FLNA binding.
  • Fig. S5. LAD1 increases EGF-inducible ERK phosphorylation and physically interacts with 14-3-3σ (SFN).
  • Fig. S6. Effects of LAD1 on specific transcripts of breast cancer cells.
  • Fig. S7. Like LAD1, SFN expression correlates with poor prognosis in breast cancer patients; FLNA expression displays distinct pathological patterns.
  • Legends for tables S1 to S3
  • Table S4. Lists of putative partners of LAD1 revealed by using either Y2H screens or proteomic analyses of coimmunoprecipitataed proteins.
  • Legends for tables S5 and S6
  • Table S7. Primers.

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Technical Details

Format: Adobe Acrobat PDF

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Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). SILAC data.
  • Table S2 (Microsoft Excel format). EGF-induced phosphorylation changes.
  • Table S3 (Microsoft Excel format). Serum-induced phosphorylation changes.
  • Table S5 (Microsoft Excel format). RNA-seq data.
  • Table S6 (Microsoft Excel format). GOrilla analysis.

[Download Tables S1 to S3 and S5 and S6]


Citation: L. Roth, S. Srivastava, M. Lindzen, A. Sas-Chen, M. Sheffer, M. Lauriola, Y. Enuka, A. Noronha, M. Mancini, S. Lavi, G. Tarcic, G. Pines, N. Nevo, O. Heyman, T. Ziv, O. M. Rueda, D. Gnocchi, E. Pikarsky, A. Admon, C. Caldas, Y. Yarden, SILAC identifies LAD1 as a filamin-binding regulator of actin dynamics in response to EGF and a marker of aggressive breast tumors. Sci. Signal. 11, eaan0949 (2018).

© 2018 American Association for the Advancement of Science