Supplementary Materials

Supplementary Materials for:

G protein–coupled receptors control the sensitivity of cells to the morphogen Sonic Hedgehog

Ganesh V. Pusapati, Jennifer H. Kong, Bhaven B. Patel, Mina Gouti, Andreas Sagner, Ria Sircar, Giovanni Luchetti, Philip W. Ingham, James Briscoe, Rajat Rohatgi*

*Corresponding author. Email: rrohatgi{at}stanford.edu

This PDF file includes:

  • Fig. S1. Cmpd101 inhibits Hh signaling in NIH/3T3 cells.
  • Fig. S2. GRK2 affects the ciliary localization of GPR161 in NIH/3T3 cells.
  • Fig. S3. GRK2 functions at a step between SMO and GαS in Hh signaling.
  • Fig. S4. SHH signaling in NPCs is mediated through GRK2/3.
  • Fig. S5. GPR161 suppresses NPC differentiation responses that are driven by low-level Hh signals.
  • Fig. S6. High-level Hh responses in Gpr161−/− NPCs depend on GRK2 and SMO.
  • Fig. S7. GαS regulates both low-level and high-level Hh responses in NPCs.
  • Table S1. List of pooled or clonal knockout cell lines generated by CRISPR/Cas9.

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Citation: G. V. Pusapati, J. H. Kong, B. B. Patel, M. Gouti, A. Sagner, R. Sircar, G. Luchetti, P. W. Ingham, J. Briscoe, R. Rohatgi, G protein–coupled receptors control the sensitivity of cells to the morphogen Sonic Hedgehog. Sci. Signal. 11, eaao5749 (2018).

© 2018 American Association for the Advancement of Science