Supplementary Materials

Supplementary Materials for:

Educated natural killer cells show dynamic movement of the activating receptor NKp46 and confinement of the inhibitory receptor Ly49A

Elina Staaf, Per Niklas Hedde, Sunitha Bagawath Singh, Joachim Piguet, Enrico Gratton, Sofia Johansson*

*Corresponding author. Email: sofia.e.johansson{at}ki.se

This PDF file includes:

  • Fig. S1. Diffusion rate and cluster size at the cell surface are not affected by antibody binding.
  • Fig. S2. Dynamic clustering of NKp46 and Ly49A at the NK cell surface.
  • Fig. S3. Simulation of diffusion with different numbers of molecules and variance of the noise.
  • Fig. S4. Fitting of simulated data sets of the different modes of movement.
  • Fig. S5. Cluster dynamics of transfected Ly49A-GFP at the cell surface is not significantly affected by antibody binding.
  • Fig. S6. Carpet iMSD analysis of Ly49A after LatB and Myovin1 treatment.
  • Fig. S7. Carpet iMSD microdomain widths after cholesterol or MβCD treatment.
  • Table S1. Simulation of diffusion with different numbers of molecules and variance of the noise.
  • Table S2. R2 of fitting simulated data to the four different models, using iMSD carpet analysis.
  • Table S3. Median R2 and percentage of cells with best fit for each model.
  • Legends for movies S1 and S2

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Format: Adobe Acrobat PDF

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mov format). Single-molecule tracking of NKp46 in H-2Dd NK cells.
  • Movie S2 (.mov format). Single-molecule tracking of Ly49A in H-2Dd NK cells.

Citation: E. Staaf, P. N. Hedde, S. Bagawath Singh, J. Piguet, E. Gratton, S. Johansson, Educated natural killer cells show dynamic movement of the activating receptor NKp46 and confinement of the inhibitory receptor Ly49A. Sci. Signal. 11, eaai9200 (2018).

© 2018 American Association for the Advancement of Science