Supplementary Materials

Supplementary Materials for:

The intracellular pyrimidine 5′-nucleotidase NT5C3A is a negative epigenetic factor in interferon and cytokine signaling

Latifa Al-Haj and Khalid S. A. Khabar*

*Corresponding author. Email: khabar{at}kfshrc.edu.sa

This PDF file includes:

  • Fig. S1. Dose-dependent induction of NT5C3A expression across different cell lines.
  • Fig. S2. Knockdown efficiency for siRNAs targeting STAT1, STAT2, and JAK1 in HeLa cells.
  • Fig. S3. Organization of NT5C3A.
  • Fig. S4. Knockdown efficiency for siRNAs targeting IRF1, IRF2, IRF3, and IRF9 in HeLa cells.
  • Fig. S5. Induction of NT5C3A expression by different inducers and the effect of NT5C3A on TNFA and CXCL1 expression.
  • Fig. S6. KEGG pathway analysis for nicotinate and nicotinamide metabolism.
  • Fig. S7. Efficiency of SIRT1 and SIRT6 knockdown.
  • Fig. S8. Protein association network (STRING).
  • Table S1. List of oligonucleotide primers.
  • Table S2. List of cloning-free primers and EMSA oligonucleotides.
  • Table S3. List of ChIP-qPCR primers.
  • Table S4. List of site-directed mutagenesis primers.
  • Table S5. List of plasmids.
  • Table S6. List of siRNA sequences.

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