Supplementary Materials

Supplementary Materials for:

Abl and Arg mediate cysteine cathepsin secretion to facilitate melanoma invasion and metastasis

Rakshamani Tripathi, Leann S. Fiore, Dana L. Richards, Yuchen Yang, Jinpeng Liu, Chi Wang, Rina Plattner*

*Corresponding author. Email: rplat2{at}uky.edu

This PDF file includes:

  • Fig. S1. Abl and Arg activities correlate with expression in melanoma cell lines.
  • Fig. S2. Effects of silencing or inhibiting Abl/Arg on intracellular and extracellular cathepsins.
  • Fig. S3. Abl/Arg drive cathepsin abundance.
  • Fig. S4. Effects of silencing or inhibiting Abl/Arg on pro, intermediate, and mature (double-chain) cathepsin forms.
  • Fig. S5. Abl and Arg promote cathepsin mRNA expression.
  • Fig. S6. Abl/Arg and cathepsin mRNAs are correlated in primary melanomas.
  • Fig. S7. Abl/Arg alter Ets1 and Sp1 protein, mRNA, and promoter activities.
  • Fig. S8. Abl/Arg alter the nuclear localization of p65, Ets1, and Sp1.
  • Fig. S9. Abl/Arg affect the DNA binding capacity of Ets1 and Sp1 on cathepsin promoters.
  • Fig. S10. Abl/Arg and Sp1 or Ets1 expression is correlated in primary melanomas.
  • Fig. S11. Ets1, Sp1, and p65 contribute to cathepsin abundance.
  • Fig. S12. Model for Abl/Arg regulation of cathepsin expression and secretion.
  • Fig. S13. Abl/Arg regulate cathepsin expression by activating NF-κB (p65).
  • Fig. S14. Abl/Arg promote invasion by inducing cathepsin L secretion.
  • Fig. S15. Abl/Arg promote lung colonization in a cathepsin L–dependent manner.
  • Table S1. qPCR primer sequences.
  • Table S2. ChIP primer sequences.

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