Supplementary Materials

Supplementary Materials for:

Convergence of Wnt, growth factor, and heterotrimeric G protein signals on the guanine nucleotide exchange factor Daple

Nicolas Aznar,* Jason Ear, Ying Dunkel, Nina Sun, Kendall Satterfield, Fang He, Nicholas A. Kalogriopoulos, Inmaculada Lopez-Sanchez, Majid Ghassemian, Debashis Sahoo, Irina Kufareva, Pradipta Ghosh*

*Corresponding author. Email: nicolas.aznar{at}inserm.fr (N.A.); prghosh{at}ucsd.edu (P.G.)

This PDF file includes:

  • Fig. S1. The GBA and PBM in Daple allosterically inhibit each other.
  • Fig. S2. RTKs and non-RTKs target distinct sets of tyrosines within the C terminus of Daple.
  • Fig. S3. Daple mediates EGF-EGFR signaling–induced activation of Gαi3.
  • Fig. S4. Phosphorylation or phosphomimic Y2025E mutation within the PBM of Daple inhibits its binding to Dvl, enhances its binding to Gαi, but has no impact on binding to FZD7R.
  • Fig. S5. Tyr phosphorylation of Daple's PBM inhibits colony growth and enhances cell migration.
  • Fig. S6. Tyr phosphorylation of Daple affects the localization and transcriptional activity of β-catenin.
  • Fig. S7. Concurrent high Daple and EGFR expression in colon cancers is associated with poor prognosis.

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