Supplementary Materials

Supplementary Materials for:

cAMPr: A single-wavelength fluorescent sensor for cyclic AMP

Christopher R. Hackley, Esteban O. Mazzoni, Justin Blau*

*Corresponding author. Email: justin.blau{at}nyu.edu

This PDF file includes:

  • Fig. S1. Comparison of cAMPr and C-G-R in ES cells.
  • Fig. S2. cAMPr excitation and emission spectra in ES cells.
  • Fig. S3. Responses of RA- and SAG-differentiated neurons to neurotransmitters.
  • Fig. S4. ATP-treated larval clock neurons respond to forskolin.
  • Fig. S5. Two-photon imaging of cAMPr and RCaMP1h in PDF-expressing LNvs.
  • Table S1. Locomotor activity rhythms of adult flies in constant darkness.
  • Table S2. Primers used for cloning.
  • Legends for movies S1 to S9

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.avi format). Time lapse of ES cells expressing cAMPr exposed to forskolin.
  • Movie S2 (.avi format). Time lapse of ES cells expressing cAMPr exposed to DMSO.
  • Movie S3 (.avi format). Time lapse of RA-differentiated neurons exposed to forskolin.
  • Movie S4 (.mov format). Time lapse of SAG-differentiated neurons exposed to forskolin.
  • Movie S5 (.avi format). Time lapse of SAG-differentiated neurons exposed to dopamine.
  • Movie S6 (.avi format). Time lapse of RA-differentiated neurons exposed to dopamine.
  • Movie S7 (.avi format). Time lapse of the response of DNs expressing cAMPr to activation of LNvs by ATP shown in pseudocolor.
  • Movie S8 (.avi format). Time lapse of the response of LNvs expressing cAMPr to activation by ATP shown in pseudocolor.
  • Movie S9 (.avi format). Time lapse of the response of LNvs expressing cAMPr and RCaMP1h to carbachol.

© 2018 American Association for the Advancement of Science