Supplementary Materials

Supplementary Materials for:

Splenic leukocytes define the resolution of inflammation in heart failure

Ganesh V. Halade,* Paul C. Norris, Vasundhara Kain, Charles N. Serhan, Kevin A. Ingle

*Corresponding author. Email: ganeshhalade{at}uabmc.edu

This PDF file includes:

  • Fig. S1. Study design and LV functional analysis after MI.
  • Fig. S2. SPM biosynthesis in the infarcted LV after MI measured by mass spectrometry.
  • Fig. S3. SPMs are reduced in the spleen after MI.
  • Fig. S4. MI-induced activation of leukocytes is associated with prostanoid generation in spleen and infarcted LV after MI.
  • Fig. S5. Macrophage depletion is associated with reduced SPMs after MI.
  • Fig. S6. Inflammatory gene expression in LV.
  • Fig. S7. Inflammatory gene expression in spleen.
  • Fig. S8. Gating strategy for flow cytometry analysis of leukocyte populations.
  • Table S1. Bioactive lipid mediator and precursor/pathway markers in no-MI and at d1 and d5 after MI in LV.
  • Table S2. Bioactive lipid mediator and precursor/pathway markers in no-MI and at d1 and d5 after MI in spleen.

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