Supplementary Materials

Supplementary Materials for:

Extension of chemotactic pseudopods by nonadherent human neutrophils does not require or cause calcium bursts

Emmet A. Francis and Volkmar Heinrich*

*Corresponding author. Email: vheinrich{at}ucdavis.edu

This PDF file includes:

  • Fig. S1. Dual-camera setup for simultaneous recording of bright-field and fluorescence images.
  • Fig. S2. Supraphysiological concentrations of C5a trigger Ca2+ bursts in resting human neutrophils without inducing chemotaxis.
  • Fig. S3. Concurrence of Ca2+ bursts and cellular contraction.
  • Fig. S4. Estimation of the cell surface area.
  • Fig. S5. Effective cortical tension during pure chemotaxis.
  • Legends for movies S1 to S4

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mp4 format). A Ca2+ burst in a human neutrophil.
  • Movie S2 (.mp4 format). Absence of Ca2+ bursts during complement-mediated, pure chemotaxis of a human neutrophil toward zymosan.
  • Movie S3 (.mp4 format). Absence of Ca2+ bursts during complement-mediated, pure chemotaxis of a human neutrophil toward β-glucan.
  • Movie S4 (.mp4 format). Supraphysiological concentrations of C5a or costimulation by shear flow can induce Ca2+ bursts in nonadherent human neutrophils.

© 2018 American Association for the Advancement of Science