Supplementary Materials

Supplementary Materials for:

TSLP signaling in CD4+ T cells programs a pathogenic T helper 2 cell state

Yrina Rochman,* Krista Dienger-Stambaugh, Phoebe K. Richgels, Ian P. Lewkowich, Andrey V. Kartashov, Artem Barski, Gurjit K. Khurana Hershey, Warren J. Leonard, Harinder Singh*

*Corresponding author. Email: yrina.rochman{at}cchmc.org (Y.R.); harinder.singh{at}cchmc.org (H.S.)

This PDF file includes:

  • Fig. S1. TSLP promotes TH2 cell differentiation without affecting the survival or proliferation of activated CD4+ T cells.
  • Fig. S2. TSLP enhances IL-4Rα abundance and promotes TH2 cell differentiation in synergy with IL-4.
  • Fig. S3. Comparative transcriptional analysis of TH2IL-4 and TH2TSLP cells.
  • Fig. S4. JAK2 inhibitor II selectively blocks the TSLP-mediated activation of STAT5.
  • Fig. S5. Comparative chromatin analysis of TH2IL-4 and TH2TSLP cells.
  • Fig. S6. TSLP-responsive CD4+ T cells, sensitized in vivo, manifest enhanced production of TH2 cytokines.
  • Legends for tables S1 to S5
  • Table S6. Primers for PCR.
  • Table S7. Primers and probes for ChIP-DNA H3K27ac.

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Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). Quantitative analysis of IL-13–positive CD4+ T cells and the amounts of secreted IL-5 and IL-13.
  • Table S2 (Microsoft Excel format). RNA-seq analysis of the gene expression profiles of TH2IL-4 and TH2TSLP cells compared to that of TH0 cells.
  • Table S3 (Microsoft Excel format). RNA-seq analysis of gene expression clusters and pathways in TH0, TH2, and TH2TSLP cells.
  • Table S4 (Microsoft Excel format). RNA-seq analysis of the gene expression profile of TH2TSLP cells compared to that of TH2IL-4 cells.
  • Table S5 (Microsoft Excel format). H3K27ac ChIP-seq tag density coordinates, 2.5-kb intervals around peak centers for shared peaks or peaks specific for TH2 and TH2TSLP cells.

[Download Tables S1 to S5]


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