Supplementary Materials for:

TSLP signaling in CD4⁺ T cells programs a pathogenic T helper 2 cell state

Yrina Rochman,* Krista Dienger-Stambaugh, Phoebe K. Richgels, Ian P. Lewkowich, Andrey V. Kartashov, Artem Barski, Gurjit K. Khurana Hershey, Warren J. Leonard, Harinder Singh*

*Corresponding author. Email: yrina.rochman{at}cchmc.org (Y.R.); harinder.singh{at}cchmc.org (H.S.)

This PDF file includes:

• Fig. S1. TSLP promotes T_H2 cell differentiation without affecting the survival or proliferation of activated CD4⁺ T cells.
• Fig. S2. TSLP enhances IL-4Rα abundance and promotes T_H2 cell differentiation in synergy with IL-4.
• Fig. S3. Comparative transcriptional analysis of T_H2^IL-4 and T_H2^TSLP cells.
• Fig. S4. JAK2 inhibitor II selectively blocks the TSLP-mediated activation of STAT5.
• Fig. S5. Comparative chromatin analysis of T_H2^IL-4 and T_H2^TSLP cells.
• Fig. S6. TSLP-responsive CD4⁺ T cells, sensitized in vivo, manifest enhanced production of T_H2 cytokines.
• Legends for tables S1 to S5
• Table S6. Primers for PCR.
• Table S7. Primers and probes for ChIP-DNA H3K27ac.

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Other Supplementary Material for this manuscript includes the following:

• Table S1 (Microsoft Excel format). Quantitative analysis of IL-13–positive CD4⁺ T cells and the amounts of secreted IL-5 and IL-13.
• Table S2 (Microsoft Excel format). RNA-seq analysis of the gene expression profiles of T_H2^IL-4 and T_H2^TSLP cells compared to that of T_H0 cells.
• Table S3 (Microsoft Excel format). RNA-seq analysis of gene expression clusters and pathways in T_H0, T_H2, and T_H2^TSLP cells.
• Table S4 (Microsoft Excel format). RNA-seq analysis of the gene expression profile of T_H2^TSLP cells compared to that of T_H2^IL-4 cells.
• Table S5 (Microsoft Excel format). H3K27ac ChIP-seq tag density coordinates, 2.5-kb intervals around peak centers for shared peaks or peaks specific for T_H2 and T_H2^TSLP cells.

[Download Tables S1 to S5]

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