Supplementary Materials
Supplementary Materials for:
The p85 isoform of the kinase S6K1 functions as a secreted oncoprotein to facilitate cell migration and tumor growth
Jianjun Zhang, Jianping Guo, Xing Qin, Bin Wang, Linli Zhang, Yingnan Wang, Wenjian Gan, Pier Paolo Pandolfi, Wantao Chen,* Wenyi Wei*
*Corresponding author. Email: chenwantao196323{at}sjtu.edu.cn (W.C.); wwei2{at}bidmc.harvard.edu (W.W.)
This PDF file includes:
- Fig. S1. Whole-proteome screen of arginine-enriched proteins.
- Fig. S2. S6K1 and S6K2 are amplified in breast cancers.
- Fig. S3. The N-terminal 6R motif facilitates PTEN secretion and cell penetration.
- Fig. S4. Purified p85, but not p70 or Δ6R-p85, enters HeLa cells.
- Fig. S5. Purified p85 enters cells to promote cell proliferation and migration.
- Fig. S6. Bacterially purified recombinant p85 enters cells to facilitate proliferation and migration of HeLa cells.
- Fig. S7. Media conditioned by p85-expressing MDA-MB-231 cells promote malignant behaviors in MEFs and WI-38 cells.
- Fig. S8. Immunodepletion of p85 suppresses malignant behaviors in MEFs induced by conditioned media.
