Supplementary Materials

Supplementary Materials for:

The p85 isoform of the kinase S6K1 functions as a secreted oncoprotein to facilitate cell migration and tumor growth

Jianjun Zhang, Jianping Guo, Xing Qin, Bin Wang, Linli Zhang, Yingnan Wang, Wenjian Gan, Pier Paolo Pandolfi, Wantao Chen,* Wenyi Wei*

*Corresponding author. Email: chenwantao196323{at}sjtu.edu.cn (W.C.); wwei2{at}bidmc.harvard.edu (W.W.)

This PDF file includes:

  • Fig. S1. Whole-proteome screen of arginine-enriched proteins.
  • Fig. S2. S6K1 and S6K2 are amplified in breast cancers.
  • Fig. S3. The N-terminal 6R motif facilitates PTEN secretion and cell penetration.
  • Fig. S4. Purified p85, but not p70 or Δ6R-p85, enters HeLa cells.
  • Fig. S5. Purified p85 enters cells to promote cell proliferation and migration.
  • Fig. S6. Bacterially purified recombinant p85 enters cells to facilitate proliferation and migration of HeLa cells.
  • Fig. S7. Media conditioned by p85-expressing MDA-MB-231 cells promote malignant behaviors in MEFs and WI-38 cells.
  • Fig. S8. Immunodepletion of p85 suppresses malignant behaviors in MEFs induced by conditioned media.

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