Supplementary Materials

Supplementary Materials for:

Hydrophobic patches on SMAD2 and SMAD3 determine selective binding to cofactors

Ken-ichi Miyazono, Saho Moriwaki, Tomoko Ito, Akira Kurisaki, Makoto Asashima, Masaru Tanokura*

*Corresponding author. Email: amtanok{at}

This PDF file includes:

  • Fig. S1. Protein constructs.
  • Fig. S2. Structure of the SMAD3-FOXH1 complex.
  • Fig. S3. Structure of the SMAD2-SKI complex.
  • Fig. S4. Superposition of SMAD structures.
  • Fig. S5. Cooperative binding of FOXH1 and SKI to SMAD2 and SMAD3.
  • Fig. S6. SMAD2- and SMAD3-binding ability of the chimeric proteins.
  • Fig. S7. Curve fitting of the SPR data in Fig. 5 and fig. S6.
  • Fig. S8. His-tag pull-down assay of the chimeric proteins with SMAD4.
  • Fig. S9. His-tag pull-down competition assay.
  • Fig. S10. Functional assay in cells.
  • Table S1. Hydrogen bonds between FOXH1 and SMAD3.
  • Table S2. Hydrogen bonds between SKI and SMAD2.
  • Table S3. Putative functions of the hydrophobic patches of SMAD2 and SMAD3.
  • Table S4. Summary of data collection and refinement statistics of the SMAD3-FOXH1 and SMAD2-SKI complexes.

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