Supplementary Materials

Supplementary Materials for:

A hierarchy of affinities between cytokine receptors and the common gamma chain leads to pathway cross-talk

Pauline Gonnord, Bastian R. Angermann, Kaitlyn Sadtler, Erin Gombos, Pascal Chappert, Martin Meier-Schellersheim,* Rajat Varma*

*Corresponding author. Email: mms{at}niaid.nih.gov (M.M.-S.); rvarma{at}xencor.com (R.V.)

This PDF file includes:

  • Fig. S1. Specificity, baseline, and kinetics of STAT activation downstream of γc cytokines.
  • Fig. S2. Expression kinetics of γc cytokine receptors as a function of TCR activation in vivo and in vitro.
  • Fig. S3. Responses in human T cells mimic those found in mouse T cells.
  • Fig. S4. STAT activation at different receptor occupancies for γc cytokines and determination of IL-7 and IL-4 dissociation constants.
  • Fig. S5. The cell surface abundance of γc is not affected by IL-4 or IL-7.
  • Fig. S6. Computational modeling reproduces experimentally determined cytokine dose-response curves and IL-7–induced suppression of IL-4–induced STAT6 phosphorylation.
  • Fig. S7. IL-7 signaling–induced phosphatase directed at STATs is not responsible for the inhibitory action of IL-7.
  • Table S1. Reported affinities of γc cytokines for their receptors.
  • Table S2. Parameter values used for simulating the affinity conversion model.
  • Table S3. Raw MFI values for receptor expression and calculated numbers of antibodies bound.
  • References (5263)

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Other Supplementary Material for this manuscript includes the following:


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