Supplementary Materials

Supplementary Materials for:

A nanoscale reorganization of the IL-15 receptor is triggered by NKG2D in a ligand-dependent manner

Štefan Bálint, Filipa B. Lopes, Daniel M. Davis*

*Corresponding author. Email: daniel.davis{at}manchester.ac.uk

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  • Fig. S1. Titration of MICA and ULBP2 concentrations for coating slides.
  • Fig. S2. The binding of NKG2D to MICA or ULBP2 does not block the binding of the NKG2D mAb 1D11 to the receptor.
  • Fig. S3. Label density variation analysis of NKG2D and IL-2/IL-15Rβ.
  • Fig. S4. Ligand-dependent reorganization of NKG2D at different times.
    Fig. S5. Fold change in the nanoscale organization of NKG2D, IL-2/IL-15Rβ, and IL-2Rα nanoclusters.
  • Fig. S6. Surface expression analysis of NKG2D and IL-2/IL-15Rβ.
  • Fig. S7. Ligand-dependent reorganization of IL-2/IL-15Rβ at different times.
  • Fig. S8. The nanoscale organization of IL-2Rα remains unaltered upon ligation of NKG2D.
  • Fig. S9. Time-dependent association between NKG2D and IL-2/IL-15Rβ nanoclusters induced by ULBP2.
  • Fig. S10. Isotype-matched control staining.

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