Supplementary Materials

Supplementary Materials for:

mTORC1 controls lysosomal Ca2+ release through the two-pore channel TPC2

Oluseye A. Ogunbayo, Jingxian Duan, Jian Xiong, Qiaochu Wang, Xinghua Feng, Jianjie Ma, Michael X. Zhu, A. Mark Evans*

*Corresponding author. Email: mark.evans{at}ed.ac.uk

This PDF file includes:

  • Fig. S1. Blind experiments on active and null hTPC2 constructs demonstrate robustness of intracellular dialysis technique.
  • Fig. S2. Na+ and Ca2+ currents mediated by endolysosomal TPC2 in response to NAADP and PI(3,5)P2.
  • Fig. S3. High and low concentrations of rapamycin suppress mTORC1 activities in HEK293 cells at different rates.
  • Fig. S4. Torin-2 induces increases in intracellular Ca2+ in HEK293 cells stably overexpressing hTPC2 and in rat pulmonary arterial myocytes.
  • Fig. S5. Torin-1 induces low-magnitude, sustained increases in intracellular Ca2+ in HEK293 cells stably overexpressing hTPC2 and in rat pulmonary arterial myocytes.

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