Supplementary Materials

Supplementary Materials for:

mTORC1 controls lysosomal Ca²⁺ release through the two-pore channel TPC2

Oluseye A. Ogunbayo, Jingxian Duan, Jian Xiong, Qiaochu Wang, Xinghua Feng, Jianjie Ma, Michael X. Zhu, A. Mark Evans*

*Corresponding author. Email: mark.evans{at}ed.ac.uk

This PDF file includes:

Fig. S1. Blind experiments on active and null hTPC2 constructs demonstrate robustness of intracellular dialysis technique.
Fig. S2. Na⁺ and Ca²⁺ currents mediated by endolysosomal TPC2 in response to NAADP and PI(3,5)P₂.
Fig. S3. High and low concentrations of rapamycin suppress mTORC1 activities in HEK293 cells at different rates.
Fig. S4. Torin-2 induces increases in intracellular Ca²⁺ in HEK293 cells stably overexpressing hTPC2 and in rat pulmonary arterial myocytes.
Fig. S5. Torin-1 induces low-magnitude, sustained increases in intracellular Ca²⁺ in HEK293 cells stably overexpressing hTPC2 and in rat pulmonary arterial myocytes.