Supplementary Materials

Supplementary Materials for:

Architecture of the complete oxygen-sensing FixL-FixJ two-component signal transduction system

Gareth S. A. Wright, Akane Saeki, Takaaki Hikima, Yoko Nishizono, Tamao Hisano, Misaki Kamaya, Kohei Nukina, Hideo Nishitani, Hiro Nakamura, Masaki Yamamoto, Svetlana V. Antonyuk, S. Samar Hasnain, Yoshitsugu Shiro,* Hitomi Sawai*

*Corresponding author. Email: yshiro{at}sci.u-hyogo.ac.jp (Y.S.); sawai{at}sci.u-hyogo.ac.jp (H.S.)

This PDF file includes:

  • Fig. S1. Purification of FixL and FixJ.
  • Fig. S2. Optical absorption spectra of full-length FixL.
  • Fig. S3. Autophosphorylation and phosphatase activities of FixL.
  • Fig. S4. SEC profiles.
  • Fig. S5. Space-filling models of FixL alone and in complex with FixJ or FixJN.
  • Fig. S6. Pseudoatomic model and SAXS curve of truncated FixL comprising PAS-A, PAS-B, and the coiled-coil region (FixLPAS-PAS).
  • Fig. S7. Experimental and simulated SAXS curves of met FixL and the FixL-FixJ complex.
  • Fig. S8. SAXS curves and pseudoatomic models of full-length FixL based on comparisons with other HKs.
  • Fig. S9. Phosphorylation activities of FixL mutants bearing mutations in the coiled-coil linker.
  • Fig. S10. Guinier plots with Pearson residuals for full-length FixL, FixLPAS-PAS, FixL-FixJ, full-length FixJ, and FixJN.
  • Table S1. Structural parameters for FixL and FixJ determined by static SAXS experiment at the BL45XU beamline at the SPring-8 synchrotron.
  • Table S2. Structural parameters for FixL and FixJ determined by SEC-SAXS at the SWING beamline at the SOLEIL synchrotron.
  • Table S3. Crystallographic statistics for full-length FixJ.

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