Supplementary Materials

Supplementary Materials for:

Chemical synapses without synaptic vesicles: Purinergic neurotransmission through a CALHM1 channel-mitochondrial signaling complex

Roman A. Romanov, Robert S. Lasher, Brigit High, Logan E. Savidge, Adam Lawson, Olga A. Rogachevskaja, Haitian Zhao, Vadim V. Rogachevsky, Marina F. Bystrova, Gleb D. Churbanov, Igor Adameyko, Tibor Harkany, Ruibiao Yang, Grahame J. Kidd, Philippe Marambaud, John C. Kinnamon, Stanislav S. Kolesnikov,* Thomas E. Finger*

*Corresponding author. Email: tom.finger{at}ucdenver.edu (T.E.F.); staskolesnikov{at}yahoo.com (S.S.K.)

This PDF file includes:

  • Fig. S1. Validation of the CALHM1 antibody.
  • Fig. S2. Localization of CALHM1 immunoreactivity in taste buds.
  • Fig. S3. Calcium responses in sensor cells.
  • Fig. S4. Effect of CBX on Mito-ID fluorescence in living taste cells.
  • Table S1. Amplitudes of calcium responses in type II taste cells from Fig. 3B.
  • Legends for movies S1 to S4

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mp4 format). 3D visualization of the relationship between a type II taste cell and the innervating nerve fiber shown in Fig. 1A.
  • Movie S2 (.mp4 format). 3D visualization of mitochondria and taste cell shown in Fig. 1B.
  • Movie S3 (.mp4 format). Relationship of atypical and typical mitochondria.
  • Movie S4 (.mp4 format). Tubular cristae in atypical mitochondria.

© 2018 American Association for the Advancement of Science