Supplementary Materials

Supplementary Materials for:

The receptor tyrosine kinase TrkB signals without dimerization at the plasma membrane

Eitan Erez Zahavi, Noam Steinberg, Topaz Altman, Michael Chein, Yuvraj Joshi, Tal Gradus-Pery, Eran Perlson*

*Corresponding author. Email: eranpe{at}post.tau.ac.il

This PDF file includes:

  • Fig. S1. Cultured SCNs specifically express TrkB.
  • Fig. S2. ACP tagging of TrkB receptor enables plasma membrane–specific labeling and maintains signaling capacity in response to BDNF.
  • Fig. S3. Comparison of TrkB and EGFR dimer or oligomer ratio by step-bleaching analysis.
  • Fig. S4. Cell-surface cross-linking of TrkA and TrkB.
  • Fig. S5. Short BDNF treatment induces cell-surface TrkB-ACP pTyr.
  • Fig. S6. Dynamin-dependent endocytosis of TrkB.
  • Fig. S7. ERK1/2 and AKT signaling in response to BDNF is mediated by Trk.
  • Fig. S8. Dynasore alone does not reduce PH-AKT-GFP signal at the plasma membrane.
  • Fig. S9. Effects of DN dynamin on ERK1/2 signaling, TrkB TIRF-FRET, and BDNF internalization.
  • Fig. S10. dSTORM and TIRF microscopy systems resolution calibration.
  • Legends for data files S1 and S2

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Other Supplementary Material for this manuscript includes the following:

  • Data file S1 (Microsoft Excel format). Mass spectrometry analysis of the 100 to 130 kDa proteome in HEK cells.
  • Data file S2 (Microsoft Excel format). Mass spectrometry analysis of the 100 to 130 kDa proteome in rodent SCNs.

[Download Data files S1 and S2]


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