Supplementary Materials

Supplementary Materials for:

Gene expression kinetics governs stimulus-specific decoration of the Salmonella outer membrane

Xinyu Hong, H. Deborah Chen, Eduardo A. Groisman*

*Corresponding author. Email: eduardo.groisman{at}yale.edu

This PDF file includes:

  • Fig. S1. LpxT protein amounts in wild-type Salmonella.
  • Fig. S2. yeiR and lpxT transcript abundances in wild-type Salmonella in low Mg2+.
  • Fig. S3. Abundance of lpxT transcript in wild-type, phoP, and lpxT promoter mutant Salmonella.
  • Fig. S4. Lipid A profiles of wild-type Salmonella in low Mg2+.
  • Fig. S5. Lipid A profiles of wild-type, lpxT promoter, pmrC, and double pmrC lpxT promoter mutant Salmonella.
  • Fig. S6. Lipid A profiles of wild-type, lpxT, pmrA, and pmrA lpxT Salmonella.
  • Fig. S7. Resistance of wild-type, pmrA, pbgP, pmrC, and lpxT Salmonella to polymyxin B.
  • Legend for table S1
  • Table S2. Coexistence of lipid A–modifying genes in the genomes of seven Gram-negative bacterial species.
  • Table S3. Bacterial strains and plasmids used in this study.
  • Table S4. Primers used in the construction of strains and plasmids.
  • Table S5. Primers used in this study for the quantification of transcripts in Salmonella.

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Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). Lipid A profiles of isogenic Salmonella strains determined by mass spectrometry.

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