Supplementary Materials

Supplementary Materials for:

Aurora B opposes PP1 function in mitosis by phosphorylating the conserved PP1-binding RVxF motif in PP1 regulatory proteins

Isha Nasa, Scott F. Rusin, Arminja N. Kettenbach,* Greg B. Moorhead*

*Corresponding author. Email: moorhead{at}ucalgary.ca (G.B.M.); arminja.n.kettenbach{at}dartmouth.edu (A.N.K.)

This PDF file includes:

  • Fig. S1. Consensus RV[S/T]F motif in PP1-interacting proteins.
  • Fig. S2. Validation of phospho-specific antibodies by dot blots and immunofluorescence.
  • Fig. S3. Validation and characterization of the RVp[S/T]F antibody.
  • Fig. S4. Phosphorylation within RVS/TF motifs during mitosis.
  • Legends for tables S1 to S6

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Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). List of peptide sequences used in this study.
  • Table S2 (Microsoft Excel format). List of proteins specifically bound to p-RV[S/T]F antibody and their binding behavior upon Aurora B inhibition.
  • Table S3 (Microsoft Excel format). Table containing SAINT analysis of the proteins enriched in the p-RV[S/T]F immunoprecipitations.
  • Table S4 (Microsoft Excel format). Table containing GO analysis based on biological processes for proteins enriched in p-RV[S/T]F immunoprecipitations.
  • Table S5 (Microsoft Excel format). Table containing GO analysis based on cellular component localization for proteins enriched in p-RV[S/T]F immunoprecipitations.
  • Table S6 (Microsoft Excel format). List of proteins containing "RV[S/T]F" motifs specifically enriched in the p-RV[S/T]F immunoprecipitation and their Aurora B sensitivity.

[Download Tables S1 to S6]


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