Supplementary Materials

Supplementary Materials for:

A DGKζ-FoxO-ubiquitin proteolytic axis controls fiber size during skeletal muscle remodeling

Jae-Sung You,* Matthew S. Dooley, Chan-Ran Kim, Eui-Jun Kim, Wei Xu, Craig A. Goodman, Troy A. Hornberger*

*Corresponding author. Email: troy.hornberger{at}wisc.edu (T.A.H.); jyou4{at}wisc.edu (J.-S.Y.)

This PDF file includes:

  • Fig. S1. Mechanical overload activates DAG-PA-mTOR signaling and increases DGKζ activity.
  • Fig. S2. DGKζ preserves type 2b fiber size during mechanical overload.
  • Fig. S3. The effects of DGKζ KO on mTOR signaling events and FoxO target gene expression after the onset of mechanical overload.
  • Fig. S4. The effects of DGKζ KO on the phosphorylation of Akt.
  • Fig. S5. The effects of DGKζ overexpression on the distribution of fiber size under various conditions.
  • Fig. S6. The effects of denervation on the distribution of fiber size in WT and DGKζ KO muscles.
  • Fig. S7. The effects of DGKζ KO on the activation of the UPS during food deprivation.
  • Fig. S8. A proposed mechanism through which DGKζ promotes skeletal muscle hypertrophy in response to mechanical overload.
  • Table S1. Muscle weight, body weight, and tibia length.

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