Supplementary Materials

Supplementary Materials for:

Tuning ITAM multiplicity on T cell receptors can control potency and selectivity to ligand density

John R. James*

*Corresponding author. Email: jj400{at}cam.ac.uk

This PDF file includes:

  • Fig. S1. Approach to evaluating the effects of synthetic receptor ITAM multiplicity.
  • Fig. S2. Effect of ITAM multiplicity on IL-2 secretion.
  • Fig. S3. Rapalog stimulates synthetic ITAM-containing receptors.
  • Fig. S4. Approach to evaluating synthetic receptor–induced Ca2+ flux.
  • Fig. S5. Kinetics of ERK phosphorylation after stimulation of synthetic receptors.
  • Fig. S6. Modeling the mechanism of ZAP-70 activation by autophosphorylation.
  • Fig. S7. Approach to evaluating the effect of ITAM multiplicity in anti-CD19 CAR–T cells.
  • Table S1. Oligonucleotide sequences.
  • Table S2. List of select reagents and resources.
  • Legend for software S1

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Other Supplementary Material for this manuscript includes the following:

  • Software S1 (.pdf format). Modeling TCR-proximal signaling with BioNetGen.

© 2018 American Association for the Advancement of Science