Supplementary Materials

Supplementary Materials for:

Dual phosphorylation of Ric-8A enhances its ability to mediate G protein α subunit folding and to stimulate guanine nucleotide exchange

Makaía M. Papasergi-Scott, Hannah M. Stoveken, Lauren MacConnachie, Pui-Yee Chan, Meital Gabay, Dorothy Wong, Robert S. Freeman, Asim A. Beg, Gregory G. Tall*

*Corresponding author. Email: gregtall{at}med.umich.edu

This PDF file includes:

  • Fig. S1. Rat Ric-8A sequence and MS coverage of tryptic and chymotryptic peptides.
  • Fig. S2. Enrichment of the IgG fraction from rabbit Ric-8A p-Ser435 antiserum.
  • Fig. S3. Stimulation of GTPγS binding to Gαi, Gαq, and Gα13 by phosphorylated and unphosphorylated Ric-8A.
  • Fig. S4. Western blotting analysis of Ric-8A aspartic acid phosphorylation site mutants.
  • Fig. S5. Validation of the CRISPR-Cas9–generated RIC-8A–null HEK293T cell line.
  • Fig. S6. Locomotion and body posture defects in C. elegans ric-8A–S472A mutants are rescued by phorbol ester.
  • Table S1. MS/MS analysis of purified recombinant Ric-8A.
  • Legend for table S2
  • Legends for movies S1 and S2

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Other Supplementary Material for this manuscript includes the following:

  • Table S2 (Microsoft Excel format). Representative CID analysis of Ric-8A to identify specific phosphorylation sites after tryptic digest and MS analysis.
  • Movie S1 (.mp4 format). C. elegans ric-8A–S472A mutant movements.
  • Movie S2 (.mp4 format). C. elegans ric-8A–S472A mutant movements after phorbol ester treatment.

© 2018 American Association for the Advancement of Science