Supplementary Materials

Supplementary Materials for:

Thrombospondin-1 promotes matrix homeostasis by interacting with collagen and lysyl oxidase precursors and collagen cross-linking sites

Silvia Rosini, Nicholas Pugh, Arkadiusz M. Bonna, David J. S. Hulmes, Richard W. Farndale, Josephine C. Adams*

*Corresponding author. Email: jo.adams{at}bristol.ac.uk

This PDF file includes:

  • Fig. S1. Identification of specific TSP1-binding peptides within the triple-helical region of collagen III.
  • Fig. S2. Detection of collagen I, TSP1, FN, LOX and collagen I C-propeptide in cell fractions, CM, and ECM isolated from HDFs.
  • Fig. S3. Detection of the association of TSP1 with collagens I and II and LOX in HDFs by in situ proximity ligation.
  • Fig. S4. TGF-β1–mediated induction of αSMA in HDFs.
  • Table S1. Primary antibodies and the dilutions used in this study.
  • Table S2. Secondary antibodies and the dilutions used in this study.
  • Table S3. Chemicals used in this study.
  • Table S4. Chemical inhibitors used in this study.
  • Table S5. Proteins used in this study.
  • Table S6. Buffers used in this study.

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