Supplementary Materials

Supplementary Materials for:

The channel-kinase TRPM7 regulates antigen gathering and internalization in B cells

Mithunah Krishnamoorthy, Laabiah Wasim, Fathima Hifza Mohamed Buhari, Tiantian Zhao, Trisha Mahtani, Josephine Ho, Sohee Kang, Francina Deason-Towne, Anne-Laure Perraud, Carsten Schmitz, Bebhinn Treanor*

*Corresponding author. Email: bebhinn.treanor{at}utoronto.ca

This PDF file includes:

  • Fig. S1. Statistical analysis of the spreading and microcluster accumulation in WT and TRPM7-KO DT40 B cells.
  • Fig. S2. Expression of TRPM7 in TRPM7-KO cells restores B cell antigen gathering.
  • Fig. S3. Statistical analysis of the spreading and microcluster accumulation in WT and TRPM7-KD DT40 B cells.
  • Fig. S4. Supplementation with extracellular Mg2+ alters signaling but not the contraction defect in TRPM7-KO and TRPM7-KD cells.
  • Fig. S5. Statistical analysis of the spreading and microcluster accumulation in PLC-γ2-T1045A DT40 B cells.
  • Fig. S6. Statistical analysis of the PIP2 accumulation in WT, TRPM7-KO, and TRPM7-KD DT40 B cells.
  • Fig. S7. Statistical analysis of the DAG accumulation in WT, TRPM7-KO, and TRPM7-KD DT40 B cells.
  • Fig. S8. Pharmacological inhibition of TRPM7 increases antigen accumulation in DT40 and primary B cells.
  • Fig. S9. Pharmacological inhibition is specific to TRPM7 channels.
  • Fig. S10. Antigen internalization is not affected in TRPM7+/− B cells.
  • Fig. S11. Supplementation with extracellular Mg2+ is not sufficient to rescue the BCR internalization defect in TRPM7-KO cells.
  • Legends for movies S1 to S5

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.avi format). B cell spreading and the contraction response are impaired in TRPM7-KO DT40 cells.
  • Movie S2 (.avi format). TRPM7 kinase activity is important for cell contraction.
  • Movie S3 (.avi format). Antigen gathering is compromised in cells expressing PLC-γ2-T1045A.
  • Movie S4 (.avi format). The amount of PIP2 at the cell-bilayer interface is altered in TRPM7-KO and TRPM7-KDcells upon activation.
  • Movie S5 (.avi format). The amount of DAG at the cell-bilayer interface is altered in TRPM7-KO and TRPM7-KD cells upon activation.

© 2018 American Association for the Advancement of Science