Supplementary Materials
Supplementary Materials for:
The channel-kinase TRPM7 regulates antigen gathering and internalization in B cells
Mithunah Krishnamoorthy, Laabiah Wasim, Fathima Hifza Mohamed Buhari, Tiantian Zhao, Trisha Mahtani, Josephine Ho, Sohee Kang, Francina Deason-Towne, Anne-Laure Perraud, Carsten Schmitz, Bebhinn Treanor*
*Corresponding author. Email: bebhinn.treanor{at}utoronto.ca
This PDF file includes:
- Fig. S1. Statistical analysis of the spreading and microcluster accumulation in WT
and TRPM7-KO DT40 B cells.
- Fig. S2. Expression of TRPM7 in TRPM7-KO cells restores B cell antigen gathering.
- Fig. S3. Statistical analysis of the spreading and microcluster accumulation in WT
and TRPM7-KD DT40 B cells.
- Fig. S4. Supplementation with extracellular Mg2+ alters signaling but not the contraction defect in TRPM7-KO and TRPM7-KD cells.
- Fig. S5. Statistical analysis of the spreading and microcluster accumulation in
PLC-γ2-T1045A DT40 B cells.
- Fig. S6. Statistical analysis of the PIP2 accumulation in WT, TRPM7-KO, and TRPM7-KD DT40 B cells.
- Fig. S7. Statistical analysis of the DAG accumulation in WT, TRPM7-KO, and TRPM7-KD
DT40 B cells.
- Fig. S8. Pharmacological inhibition of TRPM7 increases antigen accumulation in DT40
and primary B cells.
- Fig. S9. Pharmacological inhibition is specific to TRPM7 channels.
- Fig. S10. Antigen internalization is not affected in TRPM7+/− B cells.
- Fig. S11. Supplementation with extracellular Mg2+ is not sufficient to rescue the BCR internalization defect in TRPM7-KO cells.
- Legends for movies S1 to S5
Other Supplementary Material for this manuscript includes the following:
- Movie S1 (.avi format). B cell spreading and the contraction response are impaired in TRPM7-KO
DT40 cells.
- Movie S2 (.avi format). TRPM7 kinase activity is important for cell contraction.
- Movie S3 (.avi format). Antigen gathering is compromised in cells expressing PLC-γ2-T1045A.
- Movie S4 (.avi format). The amount of PIP2 at the cell-bilayer interface is altered in TRPM7-KO and TRPM7-KDcells upon activation.
- Movie S5 (.avi format). The amount of DAG at the cell-bilayer interface is altered in TRPM7-KO and TRPM7-KD cells upon activation.