Supplementary Materials

Supplementary Materials for:

Lgl reduces endosomal vesicle acidification and Notch signaling by promoting the interaction between Vap33 and the V-ATPase complex

Marta Portela, Liu Yang, Sayantanee Paul, Xia Li, Alexey Veraksa, Linda M. Parsons, Helena E. Richardson*

*Corresponding author. Email: h.richardson{at}latrobe.edu.au

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Fig. S1. Vha68-2 mutant clones show reduced Notch reporter expression, and lgl Vha68-2 mutant tissue is retained in pupal retinas.
Fig. S2. Lgl physically interacts with Vap33 and the V-ATPase component Vha44.
Fig. S3. Vap33-overexpressing tissue is retained in the pupal retina, and Vap33 mutant tissue accumulates N-intra and increases expression of the Notch reporter.
Fig. S4. Overexpression of Vap33 results in reduced bristle formation but does not suppress the NICD-induced thoracic overgrowth phenotype.
Fig. S5. Vap33 colocalizes with Vha44-GFP, and Vap33 protein abundance and localization are unaffected in lgl mutant tissue.
Fig. S6. Vap33 physically interacts with Vha68-3 and Vha68-2.
Table S1. Drosophila stocks and references.
Table S2. List of genotypes of the samples used in each figure.
Legend for data file S1

Other Supplementary Material for this manuscript includes the following: