Supplementary Materials

Supplementary Materials for:

Lgl reduces endosomal vesicle acidification and Notch signaling by promoting the interaction between Vap33 and the V-ATPase complex

Marta Portela, Liu Yang, Sayantanee Paul, Xia Li, Alexey Veraksa, Linda M. Parsons, Helena E. Richardson*

*Corresponding author. Email: h.richardson{at}latrobe.edu.au

This PDF file includes:

  • Fig. S1. Vha68-2 mutant clones show reduced Notch reporter expression, and lgl Vha68-2 mutant tissue is retained in pupal retinas.
  • Fig. S2. Lgl physically interacts with Vap33 and the V-ATPase component Vha44.
  • Fig. S3. Vap33-overexpressing tissue is retained in the pupal retina, and Vap33 mutant tissue accumulates N-intra and increases expression of the Notch reporter.
  • Fig. S4. Overexpression of Vap33 results in reduced bristle formation but does not suppress the NICD-induced thoracic overgrowth phenotype.
  • Fig. S5. Vap33 colocalizes with Vha44-GFP, and Vap33 protein abundance and localization are unaffected in lgl mutant tissue.
  • Fig. S6. Vap33 physically interacts with Vha68-3 and Vha68-2.
  • Table S1. Drosophila stocks and references.
  • Table S2. List of genotypes of the samples used in each figure.
  • Legend for data file S1

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Other Supplementary Material for this manuscript includes the following:

  • Data file S1 (Microsoft Excel format). Lgl-interacting proteins.

© 2018 American Association for the Advancement of Science