Supplementary Materials

Supplementary Materials for:

K63-linked polyubiquitin chains bind to DNA to facilitate DNA damage repair

Pengda Liu,* Wenjian Gan, Siyuan Su, Arthur V. Hauenstein, Tian-min Fu, Bradley Brasher, Carsten Schwerdtfeger, Anthony C. Liang, Ming Xu, Wenyi Wei*

*Corresponding author. Email: pengda_liu{at}med.unc.edu (P.L.); wwei2{at}bidmc.harvard.edu (W.W.)

This PDF file includes:

  • Fig. S1. K63-linked polyubiquitin chains bind free and nucleosomal DNA in vitro.
  • Fig. S2. K63-linked polyubiquitin chains bind DNA through a newly identified DIP motif including Thr9, Lys11, and Glu34 residues.
  • Fig. S3. Binding to DNA does not disrupt but rather facilitate K63-linked polyubiquitin chains in binding proteins.
  • Fig. S4. Deficiency in binding DNA leads to attenuated ability for K63-linked polyubiquitin chains in facilitating DDR.
  • Fig. S5. Cancer patient–derived ubiquitin mutants in the DIP motif display attenuated ability in repairing damaged DNA in part due to deficiency in binding DNA.
  • Table S1. Oligonucleotides.
  • Table S2. Yeast strains used in this study.
  • Reference (56)

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