Supplementary Materials
Supplementary Materials for:
K63-linked polyubiquitin chains bind to DNA to facilitate DNA damage repair
Pengda Liu,* Wenjian Gan, Siyuan Su, Arthur V. Hauenstein, Tian-min Fu, Bradley Brasher, Carsten Schwerdtfeger, Anthony C. Liang, Ming Xu, Wenyi Wei*
*Corresponding author. Email: pengda_liu{at}med.unc.edu (P.L.); wwei2{at}bidmc.harvard.edu (W.W.)
This PDF file includes:
- Fig. S1. K63-linked polyubiquitin chains bind free and nucleosomal DNA in vitro.
- Fig. S2. K63-linked polyubiquitin chains bind DNA through a newly identified DIP
motif including Thr9, Lys11, and Glu34 residues.
- Fig. S3. Binding to DNA does not disrupt but rather facilitate K63-linked polyubiquitin
chains in binding proteins.
- Fig. S4. Deficiency in binding DNA leads to attenuated ability for K63-linked polyubiquitin
chains in facilitating DDR.
- Fig. S5. Cancer patient–derived ubiquitin mutants in the DIP motif display attenuated
ability in repairing damaged DNA in part due to deficiency in binding DNA.
- Table S1. Oligonucleotides.
- Table S2. Yeast strains used in this study.
- Reference (56)
