Supplementary Materials

Supplementary Materials for:

A coupled-clock system drives the automaticity of human sinoatrial nodal pacemaker cells

Kenta Tsutsui, Oliver J. Monfredi, Syevda G. Sirenko-Tagirova, Larissa A. Maltseva, Rostislav Bychkov, Mary S. Kim, Bruce D. Ziman, Kirill V. Tarasov, Yelena S. Tarasova, Jing Zhang, Mingyi Wang, Alexander V. Maltsev, Jaclyn A. Brennan, Igor R. Efimov, Michael D. Stern, Victor A. Maltsev, Edward G. Lakatta*

*Corresponding author. Email: lakattae{at}grc.nia.nih.gov

This PDF file includes:

  • Fig. S1. Action potential repolarization failure in the transition from arrest to spontaneous action potential firing in response to β-AR stimulation.
  • Fig. S2. A schematic of the clock coupling in SANCs.
  • Table S1. Characteristics of the donor hearts used in the present study.
  • Table S2. Time-dependent evolution of action potential parameters in the transition state of an initially arrested human SANC.
  • Legends for movies S1 to S3

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mp4 format). A freshly isolated single spontaneously beating human SANC from heart 3.
  • Movie S2 (.mp4 format). LCR detection software on a spontaneously beating human SANC from heart 4.
  • Movie S3 (.mp4 format). Simultaneous measurements of membrane potential and a 2D Ca2+ signal.

© 2018 American Association for the Advancement of Science