Supplementary Materials

Supplementary Materials for:

Thyroid hormone receptor and ERRα coordinately regulate mitochondrial fission, mitophagy, biogenesis, and function

Brijesh K. Singh,* Rohit A. Sinha, Madhulika Tripathi, Arturo Mendoza, Kenji Ohba, Jann A. C. Sy, Sherwin Y. Xie, Jin Zhou, Jia Pei Ho, Ching-yi Chang, Yajun Wu, Vincent Giguère, Boon-Huat Bay, Jean-Marc Vanacker, Sujoy Ghosh, Karine Gauthier, Anthony N. Hollenberg, Donald P. McDonnell, Paul M. Yen*

*Corresponding author. Email: paul.yen{at}duke-nus.edu.sg (P.M.Y.); singhbrijeshk{at}duke-nus.edu.sg (B.K.S.)

This PDF file includes:

  • Fig. S1. ESRRA co-regulated pathways induced by TH.
  • Fig. S2. TH-ESRRA co-regulation of TCA and FAO.
  • Fig. S3. TH activated ESRRA and OXPHOS in a PPARGC1A-dependent manner.
  • Fig. S4. TH-ESRRA co-regulated the expression of genes encoding mitochondrial biogenesis factors but not those encoding mitochondrial fission, fusion, and mitophagy factors.
  • Fig. S5. TH increased ESRRA recruitment to the ULK1 gene promoter.
  • Fig. S6. DRP1 inhibition blocked the translocation of autophagic proteins but not ULK1 translocation to mitochondria.
  • Fig. S7. TH increased lysosome and mitochondria colocalization in an ESRRAdependent manner.
  • Fig. S8. ESRRA inhibition dysregulated TH-induced mitochondrial membrane potential and ROS.
  • Fig. S9. Proposed model for TH-ESRRA co-regulation of mitochondrial biogenesis, fission, mitophagy, and activity.

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Other Supplementary Material for this manuscript includes the following:

  • Data file S1 (Microsoft Excel format). THRB1 and ESRRA ChIP-seq analysis.

© 2018 American Association for the Advancement of Science