Supplementary Materials

  • This PDF file includes:
  • Fig. S1. Tomosyn silencing in RBL-2H3 cells does not modify expression of SNARE proteins.
  • Fig. S2. Tomosyn silencing in BMMCs does not modulate IL-6, TNF-α, and CCL2 secretion.
  • Fig. S3. Plasma membrane and cytosolic localization of tomosyn complexes with STX4, STX3, and SNAP-23.
  • Fig. S4. Kinetic of β-hexosaminidase release in BMMCs.
  • Fig. S5. Tomosyn/STX4 dissociation and tomosyn/STX3 association do not depend on calcium mobilization.
  • Fig. S6. PKCβ is required for mast cell degranulation but does not phosphorylate tomosyn.
  • Fig. S7. Basophil gating strategy by flow cytometry and basophil activation by immunofluorescence.
  • Fig. S8. Proposed model for tomosyn action in mast cells.
  • Table S1. Patient characteristics.
  • Table S2. List of antibodies used in the study.
  • Table S3. List of siRNAs used in the study.

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