Supplementary Materials

The PDF file includes:

• Fig. S1. Structure of the 3′ end of the wild-type VAV1 allele and the VAV1^OST allele.
• Fig. S2. Assessment of biological and technical variability across samples.
• Fig. S3. The VAV1 interactome.
• Fig. S4. The synergistic effect of CD3 and CD226 co-engagement is not observed in naïve CD4⁺ T cells.
• Legend for table S1

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Other Supplementary Material for this manuscript includes the following:

• Table S1 (Microsoft Excel format). List of proteins associated with VAV1 in resting and pervanadate-activated CD4⁺ T cells.