Supplementary Materials

This PDF file includes:

  • Fig. S1. Expression and function of MOR phosphorylation-deficient mutants.
  • Fig. S2. Inhibition of β-arrestin2 recruitment upon addition of naloxone and lack of internalization by morphine.
  • Fig. S3. β-Arrestin1 recruitment and MOR internalization upon overexpression of GRK2 WT or GRK2 DN.
  • Fig. S4. GRK3 recruitment to MOR WT and mutants, inhibition of GRK2 recruitment upon addition of naloxone, kinetics of GRK recruitment, and siRNA controls.
  • Fig. S5. Fast desensitization component of MOR.
  • Fig. S6. Patch-clamp traces for corresponding MOR mutants.
  • Table S1. Potency and maximal response of MOR mutants in cAMP assay.

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