Supplementary Materials

This PDF file includes:

  • Fig. S1. Screening for G proteins and detection of pathways activated by WT MT2.
  • Fig. S2. Predicted topology and distribution of the 40 MT2 variants.
  • Fig. S3. Detection of MT2 variants at the cell surface.
  • Fig. S4. MLT dose-response curves for Gαi1 and Gαz activation, β-arrestin2 recruitment, cAMP inhibition, and ERK activation by MT2 variants.
  • Fig. S5. G protein–dependent but β-arrestin–independent MLT-mediated ERK phosphorylation by MT2.
  • Fig. S6. Correlations between the effects of the mutations on spontaneous and agonist-promoted activity for G proteins and β-arrestin signaling parameters performed pairwise.
  • Table S1. Summary of the functional profiling of Gαi1 activation by WT MT2 and MT2 variants.
  • Table S2. Summary of the functional profiling of Gαz activation by WT MT2 and MT2 variants.
  • Table S3. Summary of the functional profiling of β-arrestin2 recruitment to WT MT2 and MT2 variants.
  • Table S4. Summary of the functional profiling of cAMP inhibition by WT MT2 and MT2 variants.
  • Table S5. Summary of the functional profiling of ERK activation by WT MT2 and MT2 variants.

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