Supplementary Materials

The PDF file includes:

  • Materials and Methods
  • Fig. S1. PCR efficiency, GC content, and sequence identity of PCR primers.
  • Fig. S2. Noise associated with each tRNA measurement for the different stress conditions.
  • Fig. S3. Biphasic response of tRNA abundance change upon exposure to stress.
  • Fig. S4. Functional enrichment of genes whose codons are better or less well adapted to stress conditions.
  • Fig. S5. Balance between anticodon supply and demand in stress conditions.
  • Fig. S6. Computed mRNA folding energy of the four different mEGFP variants.
  • Fig. S7. Plasmid map used for integrating the mEGFP variants into the yeast genome.
  • Fig. S8. mRNA abundance measurement for the mEGFP variants under stress conditions.
  • Fig. S9. Stress-induced fold changes in protein production rates during simulation.
  • Table S1. Oligos used for tRNA quantification.
  • Table S2. mEGFP variant sequences.
  • Table S3. Oligos used for mRNA quantification.
  • References (5769)

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Other Supplementary Material for this manuscript includes the following:

  • Data file S1 (Microsoft Excel format). tRNA relative abundance during the different stress conditions.
  • Data file S2 (Microsoft Excel format). Codon adaptiveness values for the different stress conditions.
  • Data file S3 (Microsoft Excel format). n-tAI and s-tAI values of genes for the different stress conditions.
  • Data file S4 (Microsoft Excel format). Summary of GO term analysis.